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Figure 2. Identification of targets for miR-100 and miR-125b in CRC cells. (A) Differentially expressed genes were identified by mining RNAseq data comparing CC, CC-CR, ΔmiR-100, ΔmiR-125b, ΔmiR-100/miR-125b cells. Venn diagram showing the overlap between genes upregulated in CC and knockout cell lines, predicted mRNA targets of miR-100 and miR-125b using three different algorithms, and association with AGO2 in CC-CR cells; (B) Heatmaps were generated from the differential RNAseq data to identify potential targets upregulated in CC cells (> 2 fold) and knockout cells (> 1.5 fold) compared to CC-CR cells; (C) Gene Ontology analysis of the targets of miR-100 and miR-125b showed enrichment in genes associated with cell migration and motility. Figure was created using ShinyGO^[37]. CRC: Colorectal cancer; CC: cystic colonies.