fig9

Figure 9. In silico recalculation of nSolver data processing. NF: Normfinder; TR: total RNA method; A: flowcharts to illustrate differences in the order of data processing steps within the three workflow subtypes; B: results of recalculations for the 4 validated miRNA changes. × indicates a nSolver automated workflow that identified a significant change in the given mRNA. Colour coding indicates the results of recalculation. green: recalculation agrees with nSolver; red: recalculation does not agree (nSolver positive, recalculation negative); blue: recalculation does not agree (nSolver negative, recalculation positive); yellow: recalculation t-test agrees but errors found in calculation.