fig8

Figure 8. Validation of miRNA expression changes. miRNA: MicroRNA; Cq: quantification cycle; A-C: target validation by miRcury RT-qPCR. Three technical replicates were performed for each of 6 biological samples per group. The mean value of each biological sample is plotted as a circle (blue: control; red: pregnant). Bars show the mean and standard deviation for each group. miRNAs are grouped as Increased or Decreased in reference to the change observed in pregnancy relative to controls in the nCounter assay. ^*P < 0.05 in 2-tailed test; ^**P < 0.005 in 2-tailed test; (^*) P < 0.05 in 1-tailed test; NS: not significant; A: ΔCq values are plotted; B: 2^-ΔCq values are plotted. Inset shows the same data plotted on a different y-axis scale; C: Fold change values are plotted. Mean expression in control groups was set to 1 for each probe. Inset shows the same data plotted on a different y-axis scale; D: Table showing the nSolver analysis results for the 4 validated changes. Workflows correctly identifying each miRNA are highlighted in yellow and marked with an X.