fig4
Figure 4. Haemolysis analysis quality control. The graph shows the ratio of miR-23-3p expression divided by miR-451a expression calculated from the difference in Cq values. Cq: Quantification cycle. The threshold indicates a ratio of 5, which is considered to indicate haemolysis. Single technical replicate per rat due to limited sample availability, 6 biological replicates per group as shown. Thus, sample preparation [Figure 1D upper box] was successful and all samples passed quality control.
