fig3

Figure 3. RNA preparation quality control - detection of spike in controls and stably expressed plasma miRNA. Thresholds were set in the exponential phase of amplification (0.01 ΔRn) and the same threshold was used for each miRNA. miRNA: MicroRNA; A-E: graphs show the quantification cycle (Cq) of the indicated miRNA for each of the 12 rats; F: table to show the percentage coefficient of variance of Cq value for each probe within each group. Single technical replicate per rat due to limited sample availability, 6 biological replicates per group as show.