fig3

Figure 3. Regression analysis between the log-transformed concentration of EVs^* (A), ApoA1 (HDL) (B), ApoB ((V)LDL and chylomicrons) (C), and proteins (D) in the pooled SEC fractions, and the cycle threshold of two EV-associated (miR-21-5p and let7a-5p) and one lipoprotein-associated (miR-122-5p) miRNA, as detected by qRT-PCR. The slopes indicate the cycle threshold change per order of magnitude increase of the concentration. Experiments were performed in triplicate using pooled plasma obtained from healthy controls. A linear regression analysis was used to quantify the relationship between the relative quantity of miRNAs and the log-transformed concentration of EVs and (lipo)proteins. ^*EVs were measured using flow cytometry (Apogee A60-Micro) and the concentration of EVs is calculated as the sum of particles that were positively labeled for CD61, CD235a, or CD45, with a size range of 200-650 nm and a refractive index < 1.42; EV: Extracellular vesicle; SEC: size-exclusion chromatography; qRT-PCR: quantitative real-time PCR.