fig2

Figure 2. The purity of EVs^* in the pooled SEC fractions, compared to ApoA1 (HDL) by ELISA (A), ApoB [(V)LDL and chylomicrons] by ELISA (B), and protein as measured by Bradford Assay (C). Experiments were performed in triplicate using pooled plasma obtained from healthy controls. Adjusted P-values (one-way ANOVA with Tukey’s multiple comparisons test) obtained from assessing the statistical differences in EV purity between SEC columns can be found in Supplementary Table 1. A P-value ≤ 0.05 was considered significant, and has been indicated with asterisks in the figure (^*P <0.05; ^**P < 0.01). ^*EVs were measured using flow cytometry (Apogee A60-Micro) and the concentration of EVs is calculated as the sum of particles that were positively labeled for CD61, CD235a, or CD45, with a size range of 200-650 nm and a refractive index < 1.42; DMC: dual-mode chromatography; EV: extracellular vesicle; SEC2B: size-exclusion chromatography 2B; SEC4B: size-exclusion chromatography 4B.