fig2

Figure 2. Comparison of the functionality of the pEV obtained from PC and MPC. (A) Representative images of wound healing assay at the moment of treating cells (0 h) and after 24 h; (B) Wound closure ratio after 24 h of wound treatment with pEV obtained from the relationship between wound closure of the treatment with pEV and its negative control using the formula detailed in the material and methods section; (C) Metabolic activity after 24 h of wound treatment with pEV, negative control cells (wounded and non-treated) were considered 100% of the metabolic activity; (D) Cytotoxicity assay of cells treated with pEV isolated from PC and MPC after 24 h of wound treatment with pEV. LDH activity evaluated on the medium of cells that were not wounded was considered 0 % cytotoxicity and LDH activity evaluated on the medium of cells treated with 1% Triton-X was considered 100% cytotoxicity. For all analyses, 10 PCs (n = 10; pooling 5 different donors each) and 9 MPCs (n = 9, pooling 50 different donors each) were used for in vitro treatments. Values represent the mean ± SEM. The differences between groups were determined using ANOVA and Student’s t-test post-hoc comparison. PC: platelet concentrate; MPC: multiple platelet concentrate; pEV: platelet lysate-derived extracellular vesicles. *P < 0.05