fig1

Figure 1. Characterization of the pEV isolated from PC and MPC. (A) Purity of pEV (number of particles per mg of protein) isolated from MPC and PC; (B) Protein concentration of pEV isolated from MPC and PC; (C) Particle size distribution diagram, comparing pEV isolated from PC (blue) and MPC (red); (D) Particle yield of pEV isolated from PC and MPC; (E) Protein/lipid ratio of pEV isolated from MPC and PC; (F) Comparison of mean normalized fluorescence of the AMC-H channel of PC and MPC pEV for different membrane markers; for each sample, three technical replicates were evaluated. Values represent the mean ± SEM. For all the analyses, 10 PCs (n = 10; pooling 5 different donors each), 9 MPCs (n = 9, pooling 50 different donors each), and 6 MPCs (n = 6, in the protein/lipid ratio) were used. Results were statistically compared by t-test. For Figure 1A - E, P-values are shown in the graph. For Figure 1F, P values for membrane markers were CD81 (P = 0.966), CD63 (P = 0.999), CD9 (P = 0.998), CD41b (P = 0.383), CD42a (P = 0.894), CD62 (P > 0.999) and CD29 (P = 0.996). PC: platelet concentrate; MPC: multiple platelet concentrate; pEV: platelet lysate-derived extracellular vesicles.