fig2
Figure 2. Dot blot analysis of qEV fractions using anti-casein antibody. (A) The representative dot blot images display the profiles of milk EV-isolated fractions after treatment with casein micelle disaggregation/removal reagents. Two microliters of each of the 20 fractions were blotted onto a nitrocellulose membrane. The membranes were then probed with antibodies specific for β-casein and developed using anti-mouse-HRP for signal detection; (B) Protein concentrations in all collected fractions measured by Pierce 660 protein assay. EDTA: ethylenediaminetetraacetic acid.
