fig2

Figure 2. Whole mouse exhaled breath condensate (EBC) collection system version 1.0. For this system, EBC is collected from two unrestrained mice roaming in a sealed glass chamber, which contains a removable metal grate that allows animals to move freely with normal postural movement. Airflow throughout the system is maintained and directed towards a condenser. The components of this mouse EBC collection system include: (A) an air pump that controls airflow (2.0 mL/min) of compressed breathing-grade air that is transported through 1/4 inch plastic tubes; (B) a one-way Balston 0.01 mic 93% airflow filter that maintains air sterility; (C) a glass mouse chamber (containing two mice); that is (D) securely sealed on both ends by caps with gaskets; connected to (E) a glass condenser sealed on both ends by caps, which is placed on ice to allow for the collection of EBC. It is estimated that ~62.5 µL of EBC can be captured from two mice within one h of collection.