fig3

Figure 3. Cell growth monitoring. (A) Glucose monitoring of ECS medium (dashed line) and in the circulating medium (solid line). The data show the average of both bioreactors; (B) Metabolomics’ results showing different metabolite concentrations in culture medium control (DMEM Ctl), cartridge medium control (Synthetic Ctl), culture media after EV production (Circulating), and ECS medium after EV production (Cartridge). The data show the average of both bioreactors; (C) Lactate dehydrogenase (LDH) activity in the ECS of both hollow fiber bioreactors (HFB1 and HFB2); (D) Total count of detached cells, live and dead, from the two bioreactors. Error bars represent SD.