fig2

Figure 2. CDEVs have the function of inhibiting the expression of PD-L1 in lung cancer cell lines. (A) Western blot results show that the addition of CDEVs can inhibit the expression of PD-L1 and p-STAT3 in HCC15, H520, and LLC cell lines; (B) Flow cytometry results show a decrease in PD-L1 expression in LLC cells after the addition of CDEVs; (C) Flow cytometry results demonstrate a significant increase in PD-1 expression in Jurkat cells after PMA stimulation; (D and E) Secreted IL-2 from Jurkat-mediated HCC15 or H520 assays was measured using flow cytometry. Data are represented as mean ± SD. LLC: Lewis lung carcinoma; CDEVs: celery-derived extracellular vesicles; HCC15: human pulmonary adenocarcinoma cell line; H520: human lung squamous cell carcinoma cell line; PD-L1: programmed cell death-Ligand 1; p-STAT3: phosphorylated signal transducer and activator of transcription 3; PMA: Phorbol 12-Myristate 13-Acetate. (n = 4; ^*P < 0.05; ^**P < 0.01; ^***P < 0.01; ^***P < 0.001).