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Figure 3. Methods for isolation of EVs from biological fluids. The most commonly used techniques are based on isolation by density (UC, DG); by size (UF, FFF, exclusion chromatography); by precipitation (hydrophilic polymers, organic solvents and commercial kits); by affinity (WB, ELISA, affinity chromatography); and with microfluidic devices. Figure created with Biorender.