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Figure 2. Loss of CH25H promotes distal metastasis in TNBC. (A) Analysis for CH25H expression from GDC and TCGA RNAseq data using UCSC Xena functional genomics explorer comparing normal tissue, primary tumor tissue, and metastatic tissue; (B) CH25H expression from GSE3797 RNAseq data set comparing 4T1.2 mammary tissue and corresponding spine metastatic tissue; (C) Kaplan-Meier curve comparing cancer-related survival of WT and Ch25h^-/- mice following EO771 primary tumor resection (n = 5); (D) Quantification of area affected by EO771 lung metastasis from H&E staining (n = 5); (E) Representative H&E staining of lungs from WT and Ch25h^-/- mice exhibiting respiratory distress after EO771 primary tumor resection. Quantitative data are represented as mean ± SEM; P values: * P < 0.05; ** P < 0.01; *** P < 0.001 and ns for not significant from Student’s t test (panels B and D), or log rank test (Panel C). TNBC: Triple-negative breast cancer; WT: wild-type; TCGA:The Cancer Genomic Atlas;