fig1

Figure 1. CD9 and CD81 have different localization patterns in osteoclasts and their precursors. Upper panels. CD9 (red, left panels) is concentrated in the plasma membrane area of unfused osteoclasts (small arrows) and of mature osteoclasts (arrowhead). CD81 (green, right panel) stains internally in unfused osteoclasts (small arrows) and in osteoclasts and many areas of cytosolic staining do not overlap. Merged image shows some overlap (yellow), but subsets of CD9 and CD81 do not co-localize. Bottom panels show a blowup of a region containing unfused osteoclasts, showing the differential distribution of CD9 and CD81 in more detail. Arrow indicates CD9 stained plasma membrane. Arrowhead indicates CD81 stained cytosol. Scale bar = 20 µm in top panels and 2.5 µm in bottom panels.