fig3

Figure 3. Cytoplasmic contribution to DCV formation (I). (A) HID-1 promotes the acidification of TGN lumen by increasing H-ATPase activity, subsequently decreasing pH value and facilitating protein aggregation; (B) The µ1A subunit of AP-1A complex is critical for the sorting of PAM-1 to immature DCVs from TGN. Proteins (yellow), Protein Aggregates (orange), PAM1 (red). HID-1: high-temperature-induced dauer formation protein 1; AP-1A: adaptor protein 1A; PAM-1: peptidylglycine α-amidating monooxygenase-1; TGN: trans-Golgi network; DCV: dense core vesicles.