fig6

Figure 6. Mistletoe EVs might mediate cell uptake of val-miR218. (A) Visualization of mistletoe EVs under TEM. (B) Size distribution of mistletoe EVs. (C) U2OS cells were seeded in 96-well plates at 1 × 10⁴ cells per well and treated with mistletoe EVs (10 μg/mL) or negative control miRNA inhibitor (NC inhibitor, 50 nM) or val-miR218 inhibitor (50 nM) or the combinations, MTT assay was performed 72 h after the treatment. Compared with blank cells, *P < 0.05; compared with cells treated with EVs, #P < 0.05. (D) U2OS cells were incubated with PKH26 stained mistletoe EVs for 24 h, and cell uptake of EVs was checked under a confocal microscope. (E) FAM-labeled val-miR218 was transfected into EVs and then incubated with U2OS cells for 24 h. The interaction of EVs and cells was checked under a confocal microscope. The cell nucleus was stained by Hoechst 33342, and the cell skeleton was stained by phalloidin. n = 3.