fig5
Figure 5. Effect of val-miR218 on cell cycle and related protein expression. (A) U2OS, (B) 143B and (C) SAOS-2 cells were seeded into 6-well plates at 2 × 105 cells per well and serum-starved for 24 h. Cells were transfected with NC miRNA or val-miR218 and re-feeded with FBS. The cell cycle was analyzed either 48 h or 72 h after transfection using PI and FACS Flow cytometry analysis. n = 3. (D) Cells were seeded into 6 cm dishes and transfected with NC miRNA or val-miR218; protein was extracted 48 h after transfection and analysis for desired protein expression by western blot. n = 3. Compared cells treated with NC miRNA, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
