fig2
Figure 2. Val-miR218 inhibits cell proliferation and induces cell apoptosis in osteosarcoma cells in a time and concentration-dependent manner. (A) Osteosarcoma cells were seeded at a density of 2 × 104 cells/ well in 8-well E-Plate (ACEA Biosciences, USA) and transfected with either val-miR218 or NC miRNA. The medium was changed 24 h after transfection. Cell proliferation was monitored by iCELLigence real-time analysis (ACEA Biosciences, USA) for a total of 7 days. (B) Cells were seeded at a density of 1×105 cells/ well in 12-well plates and transfected with either val-miR218 or NC miRNA. Cell apoptosis was determined after depicted incubation periods by Annexin V/PI and FACS Flow cytometry. n = 3. Compared cells treated with NC miRNA, ** P < 0.01.
