fig4

Figure 4. In vivo cell-derived extracellular vesicle (EV) bio-distribution in abdominal area by optical imaging (OI). (A) Representative OI images, acquired the supine position following the induction of acute kidney injury (AKI) in mice and in healthy mice treated intravenously with 200 μg of labeled cell-derived EVs (LCD-EVs) or directly labeled EVs (DL-EVs) or with an equal volume of phosphate-buffered saline (PBS) (CTL). (B) Quantification of fluorescence intensity in regions-of-interest (ROI) draw free hand in the abdominal area, expressed as the average radiance ± standard deviation (SD). Sixteen AKI mice were treated with LCD-EVs, 11 AKI mice were treated with (DL-EVs); healthy mice received the same amount of LCD- and of DL-EVs (n = 12 for LCD-EVs and n = 6 for DL-EVs). ANOVA with Newman-Keuls multicomparison test was performed. *P < 0.01 AKI DL-EV vs. all the other groups. LCD-EV, labeled EVs produced by donor cells; DL-EV, directly labeled EVs. Reprinted with permission from^[16].