fig1

Figure 1. Characterization of microglia-derived exosomes from BV2 cells. (A) Schematic representation of the isolation protocol from BV2 cells. (B) Quantification of the total number of EVs and (C) size distribution of exosomes by NTA using ZetaView. (D) Representative western blots showing the expression of exosome-specific markers (Alix, TSG101, CD9, CD63). Calnexin used as a negative control for exosomes. (E) Representative transmission electron microscopy (TEM) image of exosome particles isolated by ultracentrifugation at 100,000 g. Scale bar 100 nm. (F) Representative western blot images showing protein expression of NLRP3, pro-IL1β, mIL-β in control, Tat (50 ng/mL) or HT treated-BV2-derived exosomes. Data are presented as mean ± SEM. NLRP3: NOD-, LRR- and pyrin domain-containing protein 3; IL: interleukin; mIL: mature interleukin; Tat: trans-activator of transcription; HT: heat inactivated Tat protein.