fig6

Figure 6. Sizing and enumeration of tetraspanin-positive vesicles. All data has been adjusted for dilution of the sample onto the chip. Average of three technical replicates that were run. Particle number is quantified by the number of particles in a defined area on the antibody capture spot. (A) Interferometry-based sizing and counting of label-free EVs, normalized to MIgG control. Limit of detection is 50-200 nm. (B) Quantification of the number of CD9, CD63, and CD81-positive particles after probing particles with a cocktail of fluorescent tetraspanin antibodies. Any particle that expresses a copy of the target protein and is detected by fluorescence will be counted, regardless of size. (C) Particle count by fluorescent channel shows the number of particles on each capture spot that express each fluorescent marker.