fig5

Figure 5. Protein composition of CFS EVs and SN analyzed by the Wes system (ProteinSimple). Calculated molecular weights (in kDa) are shown in red. Pseudo-gel images of representative runs shown for (A) Tetraspanins CD9, CD63, and CD81; (B) Intercellular cell adhesion molecule (ICAM-1) and annexin A5 (ANXA5); and (C) Intracellular proteins calnexin (CANX) and GM130. For all runs, the peak signal-to-noise ratio given by the software ≥ 10. CFS: Cell-free saliva; EVs: extracellular vesicles; MW: molecular weight; EV: pooled extracellular vesicle sample; SN: pooled supernatant sample.