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![Isolation and characterization of extracellular vesicles in saliva of children with asthma](https://image.oaes.cc/5cb43951-8ce0-4ef6-b4c5-ca189fe25e31/3962.fig.5.jpg)
Figure 5. Protein composition of CFS EVs and SN analyzed by the Wes system (ProteinSimple). Calculated molecular weights (in kDa) are shown in red. Pseudo-gel images of representative runs shown for (A) Tetraspanins CD9, CD63, and CD81; (B) Intercellular cell adhesion molecule (ICAM-1) and annexin A5 (ANXA5); and (C) Intracellular proteins calnexin (CANX) and GM130. For all runs, the peak signal-to-noise ratio given by the software ≥ 10. CFS: Cell-free saliva; EVs: extracellular vesicles; MW: molecular weight; EV: pooled extracellular vesicle sample; SN: pooled supernatant sample.