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![Isolation and characterization of extracellular vesicles in saliva of children with asthma](https://image.oaes.cc/5cb43951-8ce0-4ef6-b4c5-ca189fe25e31/3962.fig.2.jpg)
Figure 2. Representative electron microscopy negative staining images of pooled CFS EVs (independent EV isolations N = 180) showing round-shaped EVs. Electron micrographs show round-shaped vesicles (red arrowheads) 16-320 nm in diameter (mean 64.95 nm). Other structures seen in the background (yellow arrowheads) could be protein aggregates. Scale bars reflect the magnification at the camera. (A-C) 100k magnification, scale bar 200 nm; (D) 50k magnification, scale bar 500 nm. CFS: Cell-free saliva; EV: extracellular vesicle.