fig1

Figure 1. Graphical overview of laboratory methods for CFS collection and EV isolation. (A) CFS collection protocol. CFS was collected from N = 180 participants of the School Inner-City Asthma Intervention Study. Aliquots of CFS were frozen at -80ºC before shipment to Columbia University for EV isolation; (B) EV isolation protocol. EVs were isolated from CFS using a polymer-based reagent, EQ, in a 2:1 CFS-to-EQ ratio. After overnight incubation, EVs were precipitated from the solution by centrifugation. CFS: Cell-free saliva; EV: Extracellular vesicle; EQ: ExoQuick-TC; SBI: System Biosciences.