fig4
![Plasma microrna quantification protocol](https://image.oaes.cc/4f592ee6-db99-4c3b-9f0a-f523324e5bd9/vp7069.fig.4.jpg)
Figure 4. Representative 2-dimensional ddPCR plots of cardiovascular disease related microRNAs. ddPCR plots are separated into 4 quadrants representing FAM(-), VIC(-) events (lower left), FAM(-), VIC(+) events (lower right), FAM(+), VIC(-) events (upper left), and FAM(+) VIC(+) events (upper right). The analysis allows for quantification of total copy numbers, as well as a ratio of the microRNA of interest specific events [FAM(+), VIC(+)] normalized to the spike-in of miR39, represented by FAM(-), VIC(+) events.