fig3

Figure 3. Schematic diagram of the multi-modular peptide-based gene transfection platform used in cystic fibrosis treatment. (A) Structures of the synthetic multi-modular peptide. (B) Structure of polyoxamine-704. (C) Structure of pDNA. (D) Schematic showing nanoparticle formation: pDNA-based ternary complex (pDNA-TC) was prepared via self-assembly of the synthetic peptide, T-704, and pDNA. (E) A suggested method for delivering pDNA-TC to the lungs. (F) The pDNA of the pDNA-TC payloads translocate into cells by passive diffusion, even though all of these nanocomplexes could cross extracellular barriers in the lung tissue thanks to T-704. By overcoming the intracellular barriers, the multi-modular peptide in ternary complexes, in contrast, allows for the effective transport of genetic payloads into target cells and organelles.