fig6

Figure 6. The human induced pluripotent stem cell derived cardiomyocyte (hiPSC-CM) patch increases left ventricular (LV) wall thickness and preserves or regenerates myocardium, or both. The Mason trichrome-stained LV cross-sections of (A, B) 6-week chronic heart failure (CHF) control rats with an infarct but no treatment, and (C, D) hiPSC-CM patch-treated CHF rats 6 weeks after coronary artery ligation (3 weeks after implantation). Healthy myocardium is represented as red-purple, collagen/scar as blue, and red blood cells as small red dots. Box insets represent an area of higher magnification. Patch treatments were single dose in all animals regardless of infarct size. The dose was based on the size of scaffold to cover most of the anterior surface of the left ventricle. Placement of the patch increases anterior wall thickness and myocyte density in the previously infarcted region^[16].