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Figure 8. UPI peptide treatment produces upregulated but leaky vessels in tumors. (A) CD31 immunofluorescent staining for LLC or U87 tumors, showing the upregulated vessels in UPI-treated tumors. Scale bar: 100 µm; (B) Control or UPI peptide-treated s.c. U87 tumor-bearing mice were perfused with FITC-dextran for 10 min, following which the mice were killed. Tumors were fixed and processed for CD31 staining. Note that vessels in UPI-treated tumors were hyper leaky. Scale bar: 100 µm. (C) Transmission electron microscopy analysis of semi-thin sections from control and UPI peptide-treated s.c. implanted U87 tumors. Dotted red lines indicate tumor vessels; blue arrows depict red blood cell leakage from tumor vessels; and red arrows indicate dying tumor cells. Scale bar: 50 μm. (D) VEGFR2 expression in control or UPI peptide-treated s.c. LLC and U87 tumors. Figure 8 is adapted with permission from ref. 19. Copyright 2015 ASCI