fig4
![Epicatechin acts synergistically with curcumin-induced cytogenotoxic effect in acute promyelocytic leukemia HL-60 cell line](https://image.oaes.cc/fba4deab-9fcc-4743-81b2-f902a328c889/2085.fig.4.png)
Figure 4. The influence of the polyphenols in this study and their combinations on the level of γ-H2AX in HL-60 cells after 20 h of incubation. (A) The level of fluorescence intensity of γ-H2AX measured by flow cytometry; (B) representative dot plots of γ-H2AX vs. DNA staining. Curcumin, quercetin and epicatechin did not influence the level of γ-H2AX at the investigated concentrations (A). However the combination of epicatechin (500 μmol/L) and curcumin (40 μmol/L) increased significantly the level of phosphorylated H2AX (A), (B). Values are means ± SEM, *P < 0.05 vs. control, #P < 0.05 vs. C40 (ANOVA, Tukey post hoc test). Cur: curcumin; Q: quercetin; EC: epicatechin; RFU: relative fluorescence units