fig2

Figure 2. Demyelinating lesions of the cortical grey matter, deep grey matter and hippocampus and white matter. Anti-PLP immunostaining of sections through the rostral-caudal extent of MS212, at coronal levels A3, P1 and P3 (A). Note the widespread demyelination of the cortical grey matter (red colour mask). Quantification of the area of demyelinated tissues revealed the total area of demyelinated cortical grey matter to exceed that of the total measured area of deep grey matter and white matter (B). Data points represent the sum of all demyelinated lesions, per region of interest per case with mean and SD depicted. Groups compared by Kruskal-Wallis and Dunn’s multiple comparison post-test. Cx: cortical; GML: grey matter lesion; WML: white matter lesion. Scale bar = 1 cm