fig1

Figure 1. Study setup and sampling protocol. Whole coronal tissue slabs sectioned along coronal planes A3, P1 and P3 were prepared at three levels from immersion fixed brains (A) (created with Biorender.com). Histology and immunohistochemistry (anti-PLP immunostaining) revealed tissue anatomy and areas of frank demyelination (B) (MS278 section A3, MS202 section P1 and MS360 section P3). Stained and mounted tissue sections were digitised and colour masks depicting demyelinating lesions involving the neocortical grey matter (red colour mask), deep grey matter and hippocampus (green colour mask) and white matter (blue colour mask) were annotated for lesion quantification (C). (B, C) Scale bar = 2 cm. PLP: proteolipid protein