fig6

Figure 6. Effects of p-aminophenol alone or in combination with the selective CB2 receptor antagonist SR144528 on lipopolysaccharide (LPS)-induced nitric oxide release. BV-2 cells were treated for 15 min with various concentrations of SR144528 (0.02, 0.5, 2 mmol/L), then incubated with different concentrations (2, 20, 50 mmol/L) of p-aminophenol for a 15 min period, before being stimulated with LPS. After 24 h, nitrite concentrations in the BV-2 cell-free supernatants were measured using the Griess assay. Data from four independent experiments are normalized against nitrite concentration in samples stimulated in the absence of inhibitors. Nitrite concentration in these samples was 19.6 ± 1.8 mmol/L. The effect of treatments was assessed by the two-way analyses of variance (ANOVA), followed by Tukey's post hoc test. *P < 0.05 and **P < 0.01, significantly different from stimulated cells incubated in the absence of SR144528 (ANOVA F = 4.17, P = 0.011); ^##P < 0.01, significantly different from stimulated cells incubated in the absence of p aminophenol (ANOVA F = 232.2, P < 0.0001). ANOVA interaction F = 0.79, P = 0.62