fig5

Figure 5. Effects of p-aminophenol alone, or in combination with the fatty acid amide hydrolase inhibitor URB597, on the lipopolysaccharide (LPS)-induced nitric oxide release. BV-2 cells were treated for 15 min with various concentrations of URB597 (0.02, 0.5, 2 mmol/L), then incubated with different concentrations (2, 20, 50 mmol/L) of p-aminophenol for a further 15 min period, before being stimulated with LPS. After 24 h, nitrite concentrations in the BV-2 cell-free supernatants were measured using the Griess assay. Data from nine independent experiments are normalized against nitrite concentration in samples stimulated in the absence of inhibitors. Nitrite concentration in these samples was 13.2 ± 1.0 mmol/L. The effect of treatments was assessed by the two-way analyses of variance (ANOVA), followed by Tukey's post hoc test. *P < 0.05 and **P < 0.01, significantly different from stimulated cells incubated in the absence of URB597 (ANOVA F = 7.87, P < 0.0001); ^#P < 0.05 and ^##P < 0.01, significantly different from stimulated cells incubated in the absence of p-aminophenol (ANOVA F = 216.2, P < 0.0001). ANOVA interaction F = 1.83, P = 0.068