fig2

Figure 2. Effects of acetaminophen (A), p-aminophenol (B), and AM404 (C) on lipopolysaccharide (LPS)-induced nitric oxide release. BV-2 cells were treated with various concentrations of drugs (2, 20, 50 mmol/L) for 15 min, then stimulated with LPS. Nitrite concentrations in BV-2 cell-free supernatants were measured after 24 h using the Griess assay. Data (means ± SEM) from 3-5 independent experiments are presented. The effects of the treatments were assessed by the one-way analyses of variance (ANOVA), followed by Dunnett's post hoc test. **P < 0.01, significantly different from stimulated control cells incubated in the absence of drugs. F and P values for the main effects of one-way ANOVA are: (A) F = 1.09, P = 0.41; (B) F = 29.33, P < 0.0001; (C) F = 50.28, P < 0.0001. Insert in (C) is a phase-contrast image of LPS-stimulated BV-2 microglia (scale bar = 200 mm)