fig02

Figure 2. LPS-induced IκBα activation. CHME-5 cells were stimulated with LPS (1 μg/mL) at 37 °C for 10-270 min. A: cytoplasmic lysates were subjected to SDS-PAGE electrophoresis and immunoblotted with p-IκBα (1:1,000), IκBα (1:1,000), and β-tubulin (1:1,000) antibodies; B: integrated density, *P < 0.02 vs. US. Image is representative of 4 independent experiments (n = 4) for each treatment group. Bars for all groups are presented as mean ± SEM. LPS: lipopolysaccharide; IκBα: inhibitor kappa b alpha; SDS-PAGE: sodium dodecyl sulfate polyacrylamide gel electrophoresis; US: unstimulated