fig7

Figure 7. Treatment of amyloid precursor protein (APP) mice with chlorpyrifos oxon (CPO) results in exaggerated inflammation. Control APP mice and mice treated with CPO were evaluated for inflammatory markers. (a) Treatment of APP mice (3-week-old) with CPO showed constitutively elevated levels of tumor necrosis factor-α (TNF-α) in the brain compared to liver and serum levels. (b) Comparison of CPO treated APP mice to control APP mice showed a significant difference in the levels of TNF-α at all-time points. (c) Comparison of TNF-α (1), interleukin-1α (IL-1α) (2) and IL-6 (3) in the various treated groups. (d) APP mice were fed CPO (1 mg/kg) and then livers, blood and brains were examined at the indicated time points for TNF-α levels. Values are expressed as mean ± standard error of the mean, n = 12 per group. *Statistically significant (P < 0.05). (e) Immunostaining for glial fibrillary acidic protein (GFAP) in control (1) and CPO treated (2) animals (neonates). Brain sections were stained with anti-GFAP antibody