fig3
![A novel method for evaluating microglial activation using ionized calcium-binding adaptor protein-1 staining: cell body to cell size ratio](https://image.oaes.cc/2773c5fa-72ca-43f8-9151-7eb3f235fa69/79.fig.3.png)
Figure 3. Potential markers for microglial activation in young and old rats with or without surgery. (a) The corrected optical density as marker for microglia activation; (b) The percentage of activated microglia (with an activation stage ≥ 3) based on visual characterization. (c) The cell body to cell size ratio (%) as marker for microglia activation. YC: young control; YS: young surgery; OC: old control; OS: old surgery. **P < 0.01 and ***P < 0.001 compared to young rats. +P < 0.05 and ++P < 0.01 compared to age matched controls