fig1

Figure 1. Illustration of the image analysis method used to quantify the morphological characteristics of microglia in ionized calcium-binding adaptor protein 1 stained sections (for clarity purposes only a small section of the original picture is shown). Left: the unprocessed pictures. Middle: all the pixels that are darker than the background are traced (red lines) to determine the total cell size of all microglia combined. Right: pixel-clusters that are above an applied staining threshold and size filter are traced (red lines) to determine the total cell body size of all microglia combined, as well as the number of microglia