fig2

Figure 2. May–Grünwald–Giemsa staining results (×1000) of the cerebral spinal fluid in Japanese encephalitis patient at different phases: (a) Initial phase – Neutrophils (black arrows) are the main inflammatory cells in the background; (b) Acute phase – A decrease in the number of neutrophils and an increase in the number of lymphocytes were noted. Furthermore, activated monocytes (black arrows) are apparent; (c) Acute phase – Plasmocytes developed in the acute phase (black arrows); (d) Convalescence – Cytology mainly shows the lymphocyte reaction (black arrows)