fig1

Figure 1. Phage Aquarius Characterization. (A) Plaque morphology of Aquarius was of variable size with clear to turbid plaques. The plate image was enhanced by 30% in brightness and contrast to show detail; (B) Transmission electron microscopy at 52000X magnification. The length of the phage tail and the diameter of the phage head were measured using the software program ImageJ^[52] and were found to be 150.3 and 58.8 nm, respectively. The presence of the long non-contractile tail and the icosahedral head are characteristic of Caudoviricetes phages; (C) SIR phenotype characterized by the growth of bacteria in the center of an area of clearing following spot inoculation of phage Aquarius on cultures of C. acnes ATCC 6919 (second row streak) and two C. acnes clinical isolates (third and fourth row streaks) during an initial host range assay. No bacterial lysis was observed for the negative control spot inoculation on E. coli (first row streak). SIR: superinfection resistance.